Part:BBa_K945002

CUP1 yeast promoter

This sequence contains the inducible transcription promoter CUP1, useful for controlled gene expression which is actively enhanced in yeasts in the presence of copper.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 55
  Illegal BamHI site found at 510
  Illegal XhoI site found at 8
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  Illegal NgoMIV site found at 36
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 529
  

Part Contributions

Team Washington 2016 has removed RFC[10] illegal restriction sites from this part as an improvement to its functionality so that it may be used in other biobricks. The part can be found here: https://parts.igem.org/Part:BBa_K2165004 as part K2165004, along with further documentation and data on it.

Promoter characterization

Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the Experience page for details.

Promoter Strength

Tec-Monterrey Ekam is working on quantitatively characterizing the functionality of this part through the use of BBa_K945004 (GFP) as a reporter, which will be finalized shortly.